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GSH exists in oxidized (GSSG) or reduced (GSH) states, and optimal GSH:GSSG ratio is crucial for cellular health

Enzyme turnover assays were performed at 37C in 96 well clear bottom plates (Genesee Scientific, #25-104) in a Synergy HTX plate reader in 200L PBS using 200M NADPH, 0.2M enzyme and a range of 4-HNE concentrations from 0 to 0.4mM

For viscous meds and denser tissue, a lower gauge number (larger bore) is usually required

These findings were not attributed to variations in food intake, which implies that the observed results were mediated by alterations in metabolic processes [1]

Byrnes was also honored as the conference's Setter and Newcomer of the Year