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(2016) conducted a systematic review of the species spread in the Mediterranean, focusing on Greece, and reported rapid colonization and considerable threats to native biodiversity and fisheries

Obesity is the leading cause of non-alcoholic fatty liver disease (NAFLD) which, along with its downstream consequencessteatohepatitis, cirrhosis, and liver canceris the most common liver disease in the world (Araujo et al., 2018)

On the other hand, the NLRP3 inflammasome induces macrophage apoptosis via caspase-8 activation (163), though to what extent this pro-apoptotic function protects against AS development is still unanswered (Figure 2B)

B12 is one piece of the energy puzzle This is worth saying clearly: B12 matters, but it's not the whole story

For clarity, corresponding regions of each sequence are highlighted as follows: FLAG tag, MTS, (GGS) 3 linker, Stop codo, Gibson overhangs