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Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma, IPVH00010), blocked for 1 h using 5% BSA in TBS0.5% Tween-20 and stained overnight with primary rabbit-anti-GCLC (Sigma-Aldrich, HPA036359) or affinity-purified rabbit-anti-GGT1 (GGT129, as previously described 63 ) 1:1,000 in BSA 2.5% overnight at 4 C

10.1016/j.ijpharm.2023.123284 149 ZakharovaL

336 patients met one or more of the exclusion criteria, leaving a total of 1075 patients included in the analysis

PON1 is an antioxidant enzyme bound to HDL particles, and it plays a significant role in determining the antioxidant, anti-inflammatory, and anti-atherogenic properties of HDL-C [127]

Future investigations might focus on their possible impact under varying experimental conditions, including nutrient availability, temporal exposure patterns, and cellular age differences