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They could not only initiate apoptosis through pathways such as NF-B, MAPK and PKC, but also initiate autophagy through AMPK/mTORC and JNK-Bcl-2/Beclin 1 ( The changes of ferrous ion concentration, lipid peroxides and ferroptosis-related proteins were the key indicators to identify the occurrence of ferroptosis ( 2+ concentration in the cells was significantly increased and the ferroptosis-related protein GPX was significantly decreased, thus contributing to the production of a large amount of ROS and lipid peroxides ( Although many chemotherapeutic drugs could induce ROS production, there was no significant change in the ferroptosis-related characteristics during cell death

10.1038/s41420-023-01608-6 84 LuoJ.XieM.HouY.MaW.JinY.ChenJ.et al (2021)

The inhibitor was dissolved in dimethyl sulfoxide and was kept at 20 C

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Mesnage R, Benbrook C, Antoniou MN