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During prenatal development, marked by early neural circuit formation, de novo methylation establishes tissue-specific patterns through DNMT1 (Costello, 2003)

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Examples of such alkynyl groups include acetylenyl (-C CH), and propargyl (-CH2C CH)

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Transglutaminase 2 crosslinks the glutathione S-transferase tag, impeding proteinprotein interactions of the fused protein

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