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Mouse husbandry For the isolation of mitochondria and proximal tubular cells, kidneys were excised from cadavers of 812-week-old male and female wild type C57/BJ and C57/BJ crossed with 129S6 used for other experimental purposes not related to this study, following the principles of the 3Rs (Replacement, Reduction and Refinement) of animal experimentation

doi: 10.1097/CM9.0000000000001364 71

The figure shows a gradual chain: intervention, flora changes, metabolite transformation, immune/inflammatory pathway readings, which eventually lead to the phytotype of rheumatoid arthritis