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doi: 10.3389/fphys.2023.1303815, 8

Identifying the virulence factors involved in hostpathogen interactions, particularly those required for K

LiuJAWaltonJCBumgarnerJRet alChronic exposure to dim light at night disrupts cell-mediated immune response and decreases longevity in aged female mice

Cumming (New York, NY: Wiley-Liss), 201215

Samples each containing approximately 500 g of protein in SDC lysis buffer (100 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid [HEPES], 1% sodium deoxycholate [SDC], pH 8.1) were reduced and alkylated using 10 mM tris(2-carboxyethyl)phosphine (TCEP Sigma Aldrich) and 40 mM of chloroacetamide (CAA) at 95 C for 5 min