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The cell viability assay showed that curcumin analogue, MS13 (1,5-Bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadien e-3-one) has a greater cytotoxicity effect on SW480 (EC50: 7.52.8 M) and SW620 (EC50: 5.72.4 M), compared with curcumin (SW480, EC50: 30.61.4 M) and SW620, EC50: 26.82.1 M)

However, the mTORC1 pathway and cell proliferation are unchanged ( 5.5 Glutamine-related metabolites regulate other metabolisms or signaling pathways As shown in Figure 2, glutamine and related metabolites not only activate the mTORC signaling pathway, but also regulate other metabolisms or signaling pathways