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Filtered viral supernatant was then concentrated by Lenti-X Concentrator (#631232, Takara) following the manufacturers instruction and the virus pellet was resuspended in 1/100th of the original volume using cold DMEM

(c) GlyNAC supplementation for eight-weeks corrected the expression of GCLC, GCLM and GSS, and increased GSH concentrations in all three organs, thereby correcting GSH deficiency

These supplements have been clinically shown to slow the progression of advanced AMD (Snodderly, 1995)

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