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Neurons were transfected with mRFP-P-actin on day in vitro 6 (DrV6) using LipofectAMINE 2000 (Invitrogen) according to the manufacturer's protocol

8.2 Endogenous repair Given the limitations of exogenous MSC/disc stem cell transplantation, strategies to activate endogenous nucleus pulposus-derived stem cells (NPMSCs) to participate in intervertebral disc degeneration (IDD) repair have become a research hotspot