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In SLE, oxidative stress inhibits ERK signaling in CD4+ T cells, decreases DNMT1 expression and induces promoter demethylation with aberrant overexpression of normally silenced genes, thus mechanistically associating inflammatory stress to erosion of the T-cell methylome (109-111)

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1.15 mM oxidized GSH in the assay buffer) was added with 10 l of sample and 30 l R2 reagent (1 mM NADPH in GR assay buffer) into a plastic cuvette

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[DOI] [PMC free article] [PubMed] [Google Scholar] 231.Tibullo D., Li Volti G., Giallongo C., Grasso S., Tomassoni D., Anfuso C.D., Lupo G., Amenta F., Avola R., Bramanti V