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Identification and relative quantitation of protein mixtures by enzymatic digestion followed by capillary reversed-phase liquid chromatographytandem mass spectrometry

Despite certain discrepancies between the predicted and the actual results, the computation tools successfully identified nanobody mutants with significantly improved thermal stability, demonstrating the feasibility of using this prediction scheme for identifying stable nanobody mutants with pAcF incorporated

FIGURE 1 To determine whether there was synergy between GSH and meropenem, the FICI for GSH in combination with meropenem was measured