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qPCR reactions were performed under the following conditions: 10 min at 95 C and 40 cycles of the one step thermal cycling of 3 s at 95 C and 30 s at 60 C in a 96-well reaction plate

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Khan FU, Nasir F, Iqbal Z, Neau S, Khan I, Hassan M, et al

A variant located within an intron of the HLA complex P5 ( HCP5 ) gene, specifically the rs3094228 variant, has been found to have a separate association with both the susceptibility to GD and the age at which the disease begins in populations of Caucasian descent [18]