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Since warfarin initially decreases protein C levels faster than the coagulation factors, it can paradoxically increase the blood's tendency to coagulate when treatment is first begun (many patients when starting on warfarin are given heparin in parallel to combat this), leading to massive thrombosis with skin necrosis and gangrene of limbs

Working with a complete setup minimizes contamination risk and ensures a smooth process

Proper fluid protocols should be established before beginning any research

The term pernicious is also an anachronism, predating the 1948 discovery of B as an effective treatment