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Chromatin immunoprecipitation assays ChIP experiments were conducted using ChIP-IT High Sensitivity Kit (ab185913, Abcam) with adaptations

10.1016/j.mtbio.2021.100139 109 YangW

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This highly invasive delivery methodology itself induces brain damage, introduces surgical risks from anesthesia and infection, and requires four separate cannulas to dose all essential terminal fields and cell body areas

They were then washed with PBS on the rotating bed for 5 min and permeabilized using 0.1% Triton X-100 in PBS for 10 min, followed by another 5 min PBS wash on the rotating bed