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During each turn of the cycle, two carbon atoms leave the cycle as CO 2 in the decarboxylation reactions catalyzed by isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase

10.1016/j.bbacli.2016.02.003 BBA Clin

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While significant progress has been made in understanding NMmicrobiome interactions using approaches such as 16S/18S rRNA amplicon sequencing for taxonomic profiling, WGS metagenomics for functional gene prediction, and culture-based assays for targeted microbial analyses, these methods offer only partial insights into microbial function

This guide reports how a 5 mg vial maps onto that range so the syringe math is clear it is not a recommended protocol, and the underlying dose is anecdotal, not trial-validated