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For the purification of His-tagged AtSNX1 and His-tagged AtSNX2a, the procedure was the same as that for full-length AtSNX1, except for slight variations in buffer compositions

The exact effects vary based on the drugs involved, the amount consumed, and whether other substances were used at the same time

Safety profiles remain based on short-term animal studies rather than lifelong human observation

Current pharmacotherapies, including tricyclic antidepressants, gabapentinoids, and serotonin norepinephrine re-uptake inhibitors, are discussed, as are emerging treatments, such as ambroxol, cannabidiol, and N-acetyl-L-cysteine

The detection and quantification of organic acids rely on a range of analytical methods, each suited to specific acid types and concentrations