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In addition, the use of two consecutive purification steps and the distinct nature of HIC and IMAC methodologies is known to help for the efficient removal of contaminating proteins (McCluskey et al., 2007)

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Might some functions be impaired

It needs to be emphasized, as final observation, that authors do not know for sure whether the modifications of the microbiota are cause, effect, or side effect of opioid addiction in the context of other hepatic diseases

Increased polyamines alter chromatin and stabilize autoantigens in autoimmune diseases