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This hypothesis-generating study indicates that short-term CR before donation is feasible and suggests that CR promotes organ protection in humans by dampening insulin signaling and inflammation, providing a basis for future targeted interventions in clinical and transplant research moving forward

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Cells were washed three times with PBS to remove unbound antibodies and then incubated with 488 fluorophore-conjugated secondary antibodies (diluted 1:400 in 1% BSA) for 1 h at room temperature in the dark

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