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Additional plateable PHHs Hum180931 (Lonza, catalog: HUCPI) were thawed and plated in hepatocyte plating medium (Lonza, catalog: MP250) overnight, then the medium was replaced with hepatocyte culture medium (Lonza, catalog: CC-3198)

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The study utilizing single-voxel proton magnetic resonance spectroscopy (1H MRS) to analyze glutamate levels relative to creatine in individuals with Primary Insomnia (PI) suggests no significant direct association between glutamate concentrations in the dorsal anterior cingulate cortex (dACC) and measures of sleep quality within subjects with Major Depressive Disorder (MDD)