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To 50 l in the wells of a 96-well plate (kept on ice), an equal volume of 2x GSH-Glo reagent containing GSH and the luciferin derivative was added with incubation for 30 min in the dark followed by 15 min with luciferin detection reagent (100 l)

Baardman, J., Licht, I., De Winther, M

ISRN Pharm

20 , 23992401.e2394 (2022)

3a), likely weakening the interaction between the catalytic core and the pHAT domains, resulting in no observed density for the pHAT domains in the cryo-EM structure of Ta OGA (Fig