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Description

The antibodies (and their dilution) used in this study were: myc (mouse Covance, rabbit Proteintech, 16286-1-AP 1/3000e), histone 3 (Abcam, 1791, 1/20,000e), GFP (Life Technologies, A11122, 1/3,000e), GST (Cell signalling, 2624, 1/5,000e), MBP (Cell signalling, 2396, 1/3,000e), nanobody-alpha-HRP (Nanotag, N1505, 1/2,000e) Protein purification and GST pull-down His-tagged and GST-tagged proteins were cloned for this study or from our previous work 21,35 in pET or pGEX-6P plasmids, respectively, and are listed in the resource table