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Interestingly, the mesenchymal shift by Day 45 suggests greater cellular complexity at later stages, potentially facilitating iHLO use in modeling liver pathologies such as fibrosis, cirrhosis, and liver remodeling, as indicated by increased expression of hepatic stellate cell markers such as DES and VIM 33,34

By combining these agents in a precise 1:1 ratio, researchers can investigate simultaneous pathways of cellular repair and tissue regeneration without the variable of mixing separate solutions

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For most first-time users, topical at 1 to 2% delivers visible results without the regulatory complications around compounded injectables

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