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Using bioinformatic methods, Zhou et al

Ryan, Harriet

Landmesser U, Dikalov S, Price SR, McCann L, Fukai T, Holland SM, Mitch WE, Harrison DG

Over 24 hours at room temperature, NAD + showed tendency to increase by approximately 25% and NADH by 125% from the baseline at collection, while NADP + remained stable and NADPH levels decreased 69% (Supplementary Figure 1a-f)

Intracellular ROS, measured as fluorescence by 2,7-dichlorodifluorescein diacetate (DCFDA), was associated with exposure to aspartame at 0.1 mM (100 M), a non-cytotoxic concentration