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Description

Briefly, cells were resuspended in enzyme-trap buffer A (100 mM NaCl, 5 mM imidazole, 20 mM HEPES, pH 7.5) and disrupted twice using a JN-3000 Plus homogenizer at 1200 v and centrifuged at 11,000 g for 1 h

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In 1983, Weitzman and colleagues reported that sleep, in particular SWS, appears to have an inhibitory influence on the HPA axis and cortisol secretion (32)

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