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Description

First, we got a 5 mm hole with a punch in Luria-Bertani (LB) agar planted with 100 L E

Szondi, D

Slides were sealed with melted Vaseline and immediately imaged on a Zeiss Observer Z1 confocal microscope

They are counted and sized by automated particle counters (light obscuration or membrane microscopy per USP ), not by holding the vial up to a light

It has been demonstrated that there is no difference in the relative efficiency of one-step and two-step systems when the expression rate of the target gene is high [3]