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Back chamber (not visible): 600 mg of pralidoxime chloride equivalent to 476.6 mg of pralidoxime in 2 mL of sterile, colorless to yellow pyrogen-free solution

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Samples were quenched in 1 mL pre-chilled 80C 80/20 methanol:water (v/v) and spiked with 50L 1M isotope labeled TCA cycle mix (Cambridge Isotope Laboratories MSK-TCA-A) pre-diluted in 80/20 methanol:water and 50L 0.02 ng/L Propionyl-L-carnitine-(N-methyl-d3) (Sigma Aldrich 52941)