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doi: 10.1016/j.biopsych.2014.01.008 168

The slides were washed in PBS and then blocked using 10% goat serum in PBS for 1 h at room temperature before adding primary antibodies diluted in the blocking buffer

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However, when APAP is ingested in excessive amounts, the capacity of GSH to neutralize NAPQI becomes overwhelmed, leading to depletion of hepatic GSH stores and accumulation of NAPQI, resulting in oxidative stress, mitochondrial dysfunction, and hepatocellular injury, ultimately culminating in acute liver injury and potential liver failure