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Based on their subcellular localization inside a mammalian cell and their structures, GSTs can be divided into three categories: cytosolic GSTs, mitochondrial GSTs (kappa-class GSTs), and microsomal GSTs (Membrane-Associated Proteins in Eicosanoid and Glutathione metabolism, MAPEG) [3, 4]

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Taking an NAC supplement with it helps by increasing cellular levels of the amino acids that are needed for glutathione production

The supernatant was collected, and protein amount in cell lysate was quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific 23225) following the manufacturers protocol

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