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Pfmdr1 Copy Number For all genotyping assays, DNA was extracted from FTA filter paper blots or whole blood (for ex vivo specimens) according to manufacturer instructions (QIAamp DNA Blood Mini Kit, QIAGEN, Inc, Alameda, California, USA)

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knockdown of the NNMT protein in the adipose tissue and liver using antisense oligonucleotides limited body weight gain in mice fed high fat diet, causing substantial fat mass reduction via increased energy expenditure