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Then cells were washed once in dPBS1X, the pellet was resuspended in 90 l secondary antibody solution (blocking buffer + 1:500 Goat anti-rabbit IgG Alexa Fluor 647 antibody cat

After the initiation of folic acid fortification, mean plasma folate concentrations increased from 4.6 to 10 ng/mL, homocysteine concentrations fell from 10.1 to 9.4 mol/L, and the prevalence of homocysteine levels above 13 mol/L dropped from about 19% to about 10%

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