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Description

The pH of each sample was adjusted within the range of 7 to 9 using 014 pH strips (#13640516, Thermo Fisher Scientific) before subjecting them to digestion with mass spectrometry-graded trypsin (#90057, Thermo Fisher Scientific) at a 1:20 trypsin to protein (w/w) ratio

Consequently, Nb1053 has been radiolabeled with 18 F, resulting in a radioconjugate excreted via the hepatobiliary system, that prevents renal accumulation without compromising tumor uptake [99,100,101,102,103,104,105,106,107,108,109]