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Additionally, mutations such as p.Thr308Met and p.Gln364Arg selectively impair Mn 2+ transport while retaining Zn 2+ uptake, indicating altered substrate specificity

elegans strains were maintained on standard NGM plates seeded with OP50 E.coli at 20 Celsius degree

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6 The quality of evidence is categorized as A (high), B (moderate), C (low), or D (very low), and recommendations are classified as strong (1) or weak (2) (Table 1)