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For mouse brains injected with AAV vectors, brain tissue was isolated and homogenised in a buffer composed of 20 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 0.5% Triton X-100, and complete protease inhibitor cocktail (EDTA-free) and sheared with a 23-G and then by a 27-G needle syringe with agitation for 1 h at 4 C

(2007) 3:e26

This can be especially helpful for active women who find that their joints feel a bit "loose" or achy right before their period begins

MeCbl was unable to inhibit the GSDME-NT-induced LDH release, further supporting that MeCbl engages GSDME but not its activated form GSDME-NT

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