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Molecular remedy of complex I defects: rotenone-insensitive internal NADH-quinone oxidoreductase of Saccharomyces cerevisiae mitochondria restores the NADH oxidase activity of complex I-deficient mammalian cells

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The sequence of reactions may be formulated as follows: (6) Enz-[Por-Fe(III)] + nH 2 O 2 = Enz-[Por+.-Fe(IV)=O] + H 2 O + (n-1)H 2 O 2 (7) Enz-[Por+.-Fe(IV)=O] + (n-1)H 2 O 2 = Enz-[Por-Fe(III)] + H 2 O + O 2 + (n-2)H 2 O + (n-2/2)O 2 Adding Equations 6 and 7 gives: (8) nH 2 O 2 = 2H 2 O + O 2 + (n-2)H 2 O + (n-2/2)O 2 Which simplifies to: (9) nH 2 O 2 = nH 2 O + n/2 O 2 Finally, doubling both sides of Equation 9 and dividing through by n, gives Equation 1: (1) 2H 2 O 2 = 2H 2 O + O 2 By these reactions effectively reducing back to Equation 1, it implies this preliminary network hypothesis presented here does not contradict the known catalase H 2 O 2 disproportionation chemistry that has previously been discovered and investigated