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The cells in liver and muscle tissues can manage glucose better, helping reduce insulin resistance

& Weiss, S

Protein preparation and Western blot analysis Cultured cells or flashfrozen tissues were homogenized in icecold radioimmunoprecipitation assay (RIPA) buffer (50 mM TrisHCl pH 7.6, 150 mM NaCl, 0.5% sodium deoxycholate, 1% NP40, 0.1% SDS, 2 mM EDTA) containing COMPLETE protease inhibitor cocktail (Roche) and phosphatase inhibitors (Santa Cruz Biotechnology)

Ethics declarations Ethics approval and consent to participate This study complies with ethical standards

Bumetanide treatment consisted of two 0.5 mg tablets per day for 3 months, given at 8:00 am and 4:00 pm