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It is well documented that the processes of cryopreservation and transplantation induce significant oxidative stress in ovarian tissue, a phenomenon closely paralleling the impact of ROS observed in cryopreserved oocytes (69, 70)

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Samples were then evaporated to dryness in Speed vac and resuspended in 500 l Velos buffer A (95% H 2 O, 5% CH 3 CN, 0.1% FA) with additional 20 l of 20% FA to ensure the samples were acidic

Teml et al., 2007)