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Vigorous agitation introduces foam and can destabilize protein and peptide solutions

doi: 10.1016/j.cell.2011.02.013 Souers AJ, Leverson JD, Boghaert ER, Ackler SL, Catron ND, Chen J, Dayton BD, Ding H, Enschede SH, Fairbrother WJ, Huang DC, Hymowitz SG, Jin S, Khaw SL, Kovar PJ, Lam LT, Lee J, Maecker HL, Marsh KC, Mason KD, Mitten MJ, Nimmer PM, Oleksijew A, Park CH, Park CM, Phillips DC, Roberts AW, Sampath D, Seymour JF, Smith ML, et al

however, with the exception of a few side effects, no serious toxicities have been recorded for them

Current Opinion in Food Science 2021

The recombinant vectors were then transfected into cells, which were cultured in medium containing 4 mM or 0 mM Gln, respectively, followed by detection of luciferase activity