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Description

At day 11 bacteria were transferred from Pi-free media into fresh Pi-replete media or into GroPCho media (0 mM Pi, 25 mM glycerophosphocholine)

From the final supernatant, prepare two dilutionsa high and a low concentration

Since the second fragment of DAT covers part of the first 22 amino acids and contains serine residue, we were concerned that it may have unknown effects on DAT