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This validation design allowed the construction of calibration curves (using the unspiked sample, SA2, and SA3) to accurately quantify the SA1 samples and to verify linearity via the coefficient of determination ( R 2 )

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Briefly, HeLa cells were incubated with 9 or 10 (15 M) at 37 C for 12 h and then washed with cold PBS to remove any remaining probe that was not taken up by the cells

doi:10.1080/03630242.2017.1296058