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Figure 2. A 25 kDa protein was expressed in E.coli and purified on an automated system using aone-step automated protocol with ProteIndex HiBond Ni-NTA Agarose 6 Fast Flow resin under native conditions. 50 mL E.coli lysate in 50 mM NaH2PO4, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL/min. The column was then washed with 100 mL 50 mM NaH2PO4, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL/min. The target protein was eluted with 50 mM NaH2PO4, 0.3 M NaCl, 250 mM imidazole. A fraction of eluate was analyzed on a SDS-PAGE. Shown were coomassie blue stains of the target proteins purified from columns of Marvelgents ProteIndex Ni-NTA 6FF (M) and Ni-Sepharose (G) resins

In this sense, the analysis examined the independent effects of blocking and bindings FRAAs on the dependent variable while controlling for the effect of each FRAA

661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively

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The peptide demonstrates remarkable stability compared to other gastric-derived compounds