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Barcodes for each sample were introduced in a first PCR, the product of which were purified using Mag-Bind TotalPure NGS beads (Omega Bio-tek) and amplified in a second PCR introducing the standard Illumina adapters

Doses and schedules would be pre-registered, and safety monitoring would track copper status (serum copper, ceruloplasmin), injection-site reactions, and any systemic effects

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Translating ROS from a complex biological variable into a clinically actionable target will require a multi-pronged, precision-based strategy