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The slides were then stained with primary antibody (in PBS, 45 min), washed with PBS (15 min) and incubated in the dark with Alexa 488-labelled second antibody (1:1,000, 45 min.), or with Cy3-labelled secondary antibodies (1:1,000, 45 min)

Calcium requirements: new estimations for men and women by cross-sectional statistical analyses of calcium balance data from metabolic studies

J.TurnerC.GfellerK

The mixture was left for 5 min and centrifuged at 12,000 rpm at 4 for 15 min

Furthermore, HSA is extensively used, therapeutically, to treat surgical blood loss, burns, shock, hypovolemia, trauma, hemorrhage, cardiopulmonary bypass, acute respiratory distress syndrome, acute and chronic liver disease, hemodialysis, nutrition support, resuscitation and hypoalbuminemia (Alexander et al., 1982