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However, unaccustomed and/or exhaustive exercise can generate excessive reactive oxygen species (ROS), leading to oxidative stress-related tissue damages and impaired muscle contractility

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coli cytoplasm, disulfide bonds don't form (reducing environment) Trx fusion + SHuffle strain enables cytoplasmic disulfide formation This can replace periplasmic expression (higher yields) or refolding (more reliable) Affinity purification: No intrinsic affinity tagtypically used as His6-Trx or Trx-Strep

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doi:10.1002/hup.1068 [DOI] [PMC free article] [PubMed] [Google Scholar] 108.Johnston C, Meyer C, Srilakshmi J