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Yeast cells were incubated with diamide or DTT, then precipitated by treatment with trichloroacetic acid (TCA), and then free thiols (i.e., reduced cysteines) were modified with the alkylating agent 4acetamido4maleimidylstilbene2,2disulfonic acid (AMS)

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doi: 10.1016/j.jneuroim.2004.02.006

As a form of vitamin B12, methylcobalamin plays a crucial role in nerve regeneration, red blood cell formation, and overall neurological health