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To activate Cre recombinase and induce Gclc deletion in tumour pieces from Gclc f/f Rosa26 creERT2 MMTV-PYMT mice, female mice were intraperitoneally injected with tamoxifen (Sigma-Aldrich, T5648) at 50160 mg kg 1 once daily for 5 consecutive days

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A growing body of evidence suggests that DTP cells are the key cellular reservoir for cancer relapse and the major non-genetic barrier for effective cancer treatment (Sharma et al., 2010