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One explanation is that radiation and radiation-induced reoxygenation generates high levels of ROS

Further, the cells were washed twice with ice cold Dulbeccos phosphate-buffered saline (DPBS) and fixed with 4% formaldehyde for 20 min and permeablized in 0.1% Triton X-100 for 10 min, washed, and mounted using Fluoromount (Sigma) for imaging

Combined treatment faces the challenge of biological toxicity: ferroptosis can kill normal cells, while ICD may trigger cytokine storms and autoimmune diseases in patients