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The Azo-tagged protein fragment (Azo-ProtG) was purified by Strep-Tactin affinity chromatography and, to remove residual impurities, the eluate was applied to a 1 mL -CD affinity column

A daily temperature fluctuation of 9 C in the oil palm forest is also more than twice as high as in primary forests 94

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Results Development of a potent and selective CerS1 inhibitor To develop isoform-selective CerS inhibitors, we started with the multiple sclerosis drug Fingolimod (FTY720, Gilenya), which is an analogue of the endogenous lipid sphingosine 28

The benzyl alcohol in BAC water ensures that any bacteria introduced during this process cannot flourish, preserving the integrity of the solution for up to 28 days