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Following blocking, membranes were incubated with primary antibodies overnight at 4C

each well was harvested on a glass fiber filter (Perkin-Elmer, Rodgau-Jgesheim, Germany) and dried

Ghim M, Pang KT, Arshad M, Wang X, Weinberg PD (2018) A novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator