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In addition to the VN, T cells, B cells, dendritic cells (DCs), and macrophages in the follicular and marginal zones of the spleen are other major sources of ACh ( via exocytosis at specialized synaptic clefts ( Figure 1 The relative concentration of ACh in humans is found to be 8 clearance mediated via stimulation of microglial nicotinic acetylcholine receptors

Casula M, Colpani O, Xie S, Catapano AL, Baragetti A

Previous studies have demonstrated that electroconvulsive shock can activate microglia, upregulate proinflammatory cytokines, and enhance OS responses [38]

(+ ve Control): LCA cell line (A549) was maintained in drug-free environment and were kept without treatment

Because it raises triglycerides and VLDL, produces inflammatory cytokines, increases blood pressure, and drives insulin resistance, visceral fat directly damages arteries and raises cardiovascular risk even in normal-weight individuals (Hall et al., 2015